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Staff Profile: David Weetman

David Weetman BSc, MSc, PhDDW Profile

Appointment: Lecturer

Areas of interest: Insect disease vector population genetics, genomics and speciation. Mechanisms and consequences of insecticide resistance. DNA-based diagnostic development and application. Molecular approaches to ecological questions in vector population biology and behaviour.

Background and qualifications:

2006-2012       IVCC and NIAID Senior PDRA, Vector Group, LSTM
1999-2006       NERC and DEFRA PDRA, University of Hull
2000               PhD School of Biological Sciences, University of Liverpool
1995               MSc Ecology, University of Wales, Bangor
1993               BSc Zoology, University of Newcastle-upon-Tyne
2013               SEDA Professional Certificate in Supporting Learning.

Teaching: LSTM courses: MSc Biology and Control of Parasites and Disease Vectors, MSc Molecular Biology of Parasites and Disease Vectors. Co-convenor of TROP741; lecturer on modules: TROP735, 739, 936, 939, 970, 942. BIOL605 (BSc). Lecturer on LSTM Diploma in Tropical Medicine & Hygiene course.

Research: I am a population geneticist with specific interest in insect vectors of disease. I joined LSTM in 2006 and with colleagues at LSTM developed the first high throughput genotyping chip for Anopheles gambiae in one of the first projects to apply genomewide association mapping techniques outside of humans and domesticated species. We now routinely genotype and sequencing at all scales, from single polymorphisms and genes to whole genome sequencing, with a particular target of development of improving methods for discovery and application of DNA diagnostic markers for phenotypes of medical importance, especially insecticide resistance. I am also very interested in applying molecular techniques to address questions in vector population ecology and behaviour and to assess the efficacy of control methods. Prior to my arrival at LSTM, I worked in the Molecular Ecology and Fisheries Genetics Laboratory at Hull University on applied population genetics of marine and freshwater invertebrates and fishes. The work utilised molecular markers (especially microsatellites and AFLPs) to examine direct and indirect human impacts on the connectivity and diversity of natural populations. My work also involved morphometric analyses, particularly geometric morphometrics.

Representative current research

(a) Genomewide association studies of insecticide resistance [with Chris Clarkson and Martin Donnelly (LSTM), KCMC (Moshi, Tanzania), (NIMR Muheza, Tanzania), Dan Neafsey (Broad Institute, Harvard)]

(b) Role of gene duplication in Anopheles gambiae insecticide resistance dynamics [with Luc Djogbenou (Université d'Abomey-Calvi, Cotonou, Benin), John Essandoh (University of Cape Coast), Martin Donnelly (LSTM)]

(c) Anopheles gambiae speciation [with Beniamino Caputo and Alessandra della Torre (University of Rome La Sapienza); Joao Pinto (University of Lisbon); Muzamil Mahdi (University of Khartoum); Kwiatkowski group (WTSI)]

(d) Dry season dynamics of sahelian Anopheles gambiae populations [with Tovi Lehman, Diana Huestris (NIAID, Maryland)]

(e) Mechanisms of insecticide resistance in the visceral leishmaniasis vector Phlebotomus argentipes. [with Mark Paine (LSTM), Pradeep Das (RMRI Bihar)]

(f) Forensic vector blood meal analysis [with Florence Crombe]

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Techniques applied: Whole genome sequence analysis; SNP genotyping (Illumina, Affymetrix, Sequenom, TaqMan); gene expression microarrays; qRT-PCR; microsatellites; Sanger sequencing; bioassays; geometric morphometrics; genomewide association  analyses; QTL mapping.


 

Current staff and students

2014-  Florence Crombe. PDRA (MRC funded) Forensic analysis of mosquito blood

2006- Keith Steen Research technician  (NIAID funded)

2012-  PhD (LSTM funded) Chris Clarkson. Insecticide resistance and speciation in An. gambiae (with Martin Donnelly, LSTM)

2011-  PhD (AVECNet funded) Edi Constant. Mechanisms of multiple insecticide resistance in southern Cote d’Ivoire (with Hilary Ranson and Benjamin Koudou, LSTM)

2013 -  PhD (MRC funded) Kevin Opondo. Role of the vector in malaria hotspots (with Martin Donnelly, LSTM; Umberto d’Allessandro, MRC The Gambia)

2011 – PhD Paul Howell. Genetics of dieldrin resistance in Anopheles arabiensis (with Martin Donnelly, LSTM; EllenDotson, CDC).

2014: MSc Phil Dyer. Transcription of duplicated genes in An. gambiae.

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Past students

2011-2013: MSc/RA John Essandoh (University of Cape Coast). Population genetics of insecticide resistance (with Alex Egyir Yawson BNARI, Accra)

2013: MSc Annabel Murphy. Mechanisms of target site resistance to permethrin and bendiocarb in Culex quinquefasciatus from Mwanza, Tanzania.

2011: MSc Ian Smith. Testing markers for permethrin resistance in wild populations of Culex spp. from southern Ghana (with Alex Egyir Yawson BNARI, Accra)

2010-11: MSc Deodatus Maliti Population genetics of Anopheles arabiensis in Tanzania (with Hilary Ranson, LSTM; Gerry Killeen, LSTM/Ifakara)

2010: MSc Henry Mawejje Target site mediated insecticide resistance in Anopheles gambiae M and S forms from southern Ghana (with Alex Egyir Yawson BNARI, Accra)

2008: MSc Marina Cartaxo (Fiocruz, Recife, Brasil). Temporal genetic variation in Culex quinquefasciatus subjected to a vector control programme in Recifé, Brazil (with Constancia Ayres (Fiocruz, Recife, Brasil)

Current/ recent funding

LSTM PhD studentship 2012-2014 (Chris Clarkson; co-supervision with Martin Donnelly, LSTM)

NIAID 2009-2014 ($1.7M ). Development of a Field Applicable Screening Tool (FAST) kit for resistance to public health insecticides PI: Martin Donnelly, Co-Is: David Weetman, Hilary Ranson

Wellcome Trust 2011-2013 (£93k). Population genetics and resistance to alternative insecticides in southern Ghana. PI: David Weetman, Co-Is: Alexander Egyir Yawson, Hilary Ranson, research student: John Essandoh.

Asian Pacific Malaria Elimination Network travel fellowship to Zhu Guoding (LSTM visit 2013)

LSTM PhD and Research Development Funds (2012-2015)

Other LSTM responsibilities

Member of LSTM Research Ethics Committee

Member of Vector Biology Department communications team

Organiser of Vector Biology Department journal club

Advisory panel member for three current LSTM PhD students

Esteem indicators

Invited lecturer: University of Salford 2014

Invited seminar speaker: University of Cape Coast, Ghana, 2014

Invited speaker, session organiser and chair: Society of Vector Ecology, La Quinta, 2013

Invited seminar speaker: Institute of Integrative Biology, University of Liverpool, 2013

Session chair and speaker: American Society of Tropical Medicine and Hygiene, Washington, 2013

Internal PhD examiner (LSTM); MSc examiner (University College Dublin)

Member of Anopheles gambiae 1000 genomes consortium (Wellcome Trust Sanger Institute)

Regular/ recent peer reviewer for 20 international journals and funding bodies including Molecular Ecology, Parasites & Vectors, PLoS One, PNAS, the Wellcome Trust.

Selected publications

Weetman D, Wilding CS, Steen K, Pinto J, Donnelly MJ (2012) Gene flow-dependent genomic divergence between Anopheles gambiae M and S forms. Mol Biol Evol. 29:279-91.

Edi CV, Koudou BG, Jones CM, Weetman D, Ranson H (2012) Multiple-insecticide resistance in Anopheles gambiae mosquitoes, Southern Côte d'Ivoire. Emerg Infect Dis. 18:1508-11

Jones CM, Liyanapathirana M, Agossa FR, Weetman D, Ranson H, Donnelly MJ, Wilding CS (2012) Footprints of positive selection associated with a mutation (N1575Y) in the voltage-gated sodium channel of Anopheles gambiae. Proc Natl Acad Sci USA. 109:6614-9

Cartaxo MF, Ayres CF, Weetman D (2011) Loss of genetic diversity in Culex quinquefasciatus targeted by a lymphatic filariasis vector control program in Recife, Brazil. Trans R Soc Trop Med Hyg. 105:491-9

Weetman D*, Wilding CS*, Steen K, Morgan JC, Simard F, Donnelly MJ (2010) Association mapping of insecticide resistance in wild Anopheles gambiae populations: major variants identified in a low-linkage disequilbrium genome. PLoS ONE 5:e13140

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Recent Publications

Jump to: Article
Number of items: 30.

Weetman, David, Mitchell, Sara N., Wilding, Craig S., Birks, Daniel P., Yawson, Alexander E., Essandoh, John, Mawejje, Henry D., Djogbenou, Luc S., Steen, Keith, Rippon, Emily, Clarkson, Christopher S., Field, Stuart G., Rigden, Daniel J. and Donnelly, Martin (2015) 'Contemporary evolution of resistance at the major insecticide target site geneAce-1by mutation and copy number variation in the malaria mosquito Anopheles gambiae'. Molecular Ecology, Vol 24, Issue 11, pp. 21656-2672.

Weetman, David and Donnelly, Martin (2015) 'Evolution of insecticide resistance diagnostics in malaria vectors'. Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol 109, Issue 5, pp. 291-293.

Weetman, David and Clarkson, Christopher S. (2015) 'Evolving the world's most dangerous animal'. Trends in Parasitology, Vol 31, Issue 2, pp. 39-40.

Edi, Constant V.A., Koudou, Benjamin, Bellai, Louise, Adja, Akre M, Chouaibou, Mouhamadou, Bonfoh, Bassirou, Barry, Sarah, Johnson, Paul, Müller, Pie, Dongus, Stefan, N’Goran, Eliezer K, Ranson, Hilary and Weetman, David (2014) 'Long-term trends in Anopheles gambiae insecticide resistance in Côte d’Ivoire'. Parasites & Vectors, Vol 7, e500.

Maliti, Deodatus, Ranson, Hilary, Magesa, Stephen, Kisinza, William, Mcha, Juma, Haji, Khamis, Killeen, Gerry and Weetman, David (2014) 'Islands and Stepping-Stones: Comparative Population Structure of Anopheles gambiae sensu stricto and Anopheles arabiensis in Tanzania and Implications for the Spread of Insecticide Resistance'. PLoS ONE, Vol 9, Issue 10, e110910.

More...



Selected publications

(*joint first author; also Clarkson and Weetman are co-first authors on the Nature Communications paper in Recent Publications section above, and Edi and Djogbenou are co-first authors on the PLoS Genetics paper)

Weetman D, Wilding CS, Steen K, Pinto J, Donnelly MJ (2012) Gene flow-dependent genomic divergence between Anopheles gambiae M and S forms. Mol Biol Evol. 29:279-91.

Edi CV, Koudou BG, Jones CM, Weetman D, Ranson H (2012) Multiple-insecticide resistance in Anopheles gambiae mosquitoes, Southern Côte d'Ivoire. Emerg Infect Dis. 18:1508-11

Jones CM, Liyanapathirana M, Agossa FR, Weetman D, Ranson H, Donnelly MJ, Wilding CS (2012) Footprints of positive selection associated with a mutation (N1575Y) in the voltage-gated sodium channel of Anopheles gambiae. Proc Natl Acad Sci USA. 109:6614-9

Cartaxo MF, Ayres CF, Weetman D (2011) Loss of genetic diversity in Culex quinquefasciatus targeted by a lymphatic filariasis vector control program in Recife, Brazil. Trans R Soc Trop Med Hyg. 105:491-9

Weetman D*, Wilding CS*, Steen K, Morgan JC, Simard F, Donnelly MJ (2010) Association mapping of insecticide resistance in wild Anopheles gambiae populations: major variants identified in a low-linkage disequilbrium genome. PLoS ONE 5:e13140

Selected non-tropical medicine publications

Mariani S*, Peijnenburg, KCJ*, Weetman D. (2012) Independence of neutral and adaptive divergence in a low dispersal marine mollusc. Marine Ecology Progress Series 446: 173-187.

Herborg, L-M, Weetman, D, van Oosterhout, C & Hänfling, B (2007) Genetic population structure and contemporary dispersal patterns of a recent European invader, the Chinese mitten crab, Eriocheir sinensis. Mol. Ecol. 16: 231-242

Hänfling, B & Weetman, D (2006) Concordant genetic estimators of migration reveal anthropogenically-enhanced source-sink population structure in the river sculpin, Cottus gobio. Genetics, 173: 1487-1501

Van Oosterhout, C, Weetman, D & Hutchinson, WF (2006) Estimation and adjustment of microsatellite null alleles in non-equilibrium populations. Molecular Ecology Notes 6: 255-256

Weetman, D, Hauser, L & Carvalho, GR (2002) Reconstruction of microsatellite mutation history reveals a strong and consistent deletion bias in invasive clonal snails, Potamopyrgus antipodarum. Genetics, 162: 813-822